Invitrogen™ Human PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha), His Tag Recombinant Protein

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. This PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein (full length) was expressed in insect cells. PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service. : Validated for use with the Adapta™ assay: Available in multiple pack sizes for convenient use: Also available in our SelectScreen Kinase Profiling Service Protein Form: Full Length, Recombinant, Active, Mutant The gene accession number for PIK3CA (p110α) is NP_006209.2 and for PIK3R1 (p85α) is NP_852664.1.

PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) lipid kinase is comprised of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. PIK3CA is frequently mutated or amplified in a variety of cancers including ovarian and colon and this kinase is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of PIK3CA is essential for vascular development and inactivation of PIK3CA leads to severe defects in angiogenic sprouting and vascular remodeling.

Specifications

• Accession Number: P27986, P42336
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM sodium phosphate with 0.25mM DTT, 25% glycerol, 150mM Imidazole, 300mM NaCl, 0.1mM PMSF and no preservative; pH 7
• Gene ID (Entrez): 5290, 5295
• Molecular Weight (g/mol): 125.4-84.7 kDa
• Name: Human PIK3CA E545K/PIK3R1 (p110 alpha E545K/p85 alpha), His Tag
• pH Range: 7
• Purification Method: Purified
• Quantity: 20 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: PIK3CA; PIK3R1
• Common Name: PIK3CA (E545K Mutant)/PIK3R1
• Gene Symbol: PIK3CA, PIK3R1
• KinaseFamily: PI3 Kinase
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: His-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length, Recombinant, Active, Mutant
• Form: Liquid