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Thermo Scientific Chemicals PIPES, 1.0M buffer soln., pH 7.5

Catalog No. AAJ62494AP
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Catalog No. AAJ62494AP Supplier Thermo Scientific Chemicals Supplier No. J62494AP
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Description

CAS: 5625-37-6 | C8H18N2O6S2 | 302.36 g/mol

PIPES (Piperazine-N,N?-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
PIPES (Piperazine-N,N′-bis(2-ethanesulfonic acid), is frequently used as a buffering agent in biochemistry. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues and has a negligible capacity to bind divalent ions. Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer is done.

Solubility
Soluble in 1M NaOH (0.5 M at 20°C), water (∼1 g/L at 100°C), and 0.5 N NaOH (0.1 M).

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away from strong oxidizing agents.
TRUSTED_SUSTAINABILITY

Chemical Identifiers

CAS 5625-37-6
Molecular Formula C8H18N2O6S2
Molecular Weight (g/mol) 302.36
MDL Number MFCD00006159
InChI Key IHPYMWDTONKSCO-UHFFFAOYSA-N
PubChem CID 79723
ChEBI CHEBI:44933
IUPAC Name 2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
SMILES C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O

Specifications

Color Colorless
Concentration 1.0 M
pH 7.5
Physical Form Liquid
Quantity 500 mL
Solubility Information Soluble in 1M NaOH (0.5 M at 20°C),water (∽1g/L at 100°C),and 0.5 N NaOH (0.1 M).
Chemical Name or Material PIPES buffer soln.

Safety and Handling

TSCA Yes
Recommended Storage Keep cold
SDS

RUO – Research Use Only

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