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pJTI™ R4 CMV-TO MCS pA Vector Non-distribution product as customer accommodation.

pJTI™ R4 CMV-TO MCS pA Vector

Manufacturer:  LIFE TECHNOLOGIES A15004

Catalog No. A15004


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Description

Description

The pJTI™ R4 CMV-TO MCS pA vector is designed for the expression of your gene of interest under the control of the tet-inducible CMV promoter after restriction enzyme cloning and retargeting into the genomic R4 site of a Jump-In™ parental cell line.

This vector can be used for inducible or constitutive expression of your gene of interest, depending on which Jump-In™ parental cell line you use. When this vector is retargeted into a Jump-In™ T-REx™ parental cell line, gene expression is controlled by the tet-operon and can be induced by adding doxycycline to the growth media. When used with a Jump-In™ parental cell line such as the Jump-In™ GripTite™ HEK293 cell line, constitutive expression is achieved after retargeting. The R4 sites in the Jump-In™ parental cell lines result in a high retargeting efficiency, requiring less effort and less time than traditional cell engineering methods. Retargeting of Jump-In™ parental cell lines results in creation of an isogenic pool that is sufficient for cell-based experiments without the need for clonal selection. Alternatively, the high retargeting efficiency allows for easy selection of a positive stable clone for expressing your gene of interest.
Specifications

Specifications

Inducible
Liquid
Tetracycline
pJTI™ R4 CMV-TO MCS pA vector
CMV/TO
Geneticin™ (G-418)
Jump-In™ Vectors
TE buffer, (pH 8.0)
100μL
1.5μg/μL
pJTI™ R4 CMV-TO MCS pA Vector, 100μg, Liquid, CMV/TO Promoter, Transfection Delivery Method, Untagged, Restriction Enzyme //MCS Cloning Method, Jump-In Vectors, Inducible System, Tetracycline Inducing Agent, Used in Protein Expression
Protein biology, protein expression, mammalian expression, regulated expression, targeted integration, stable cell line development
Ampicillin
pJTI™ R4 CMV-TO MCS pA Vector
100μg
–20°C
Untagged
Documents
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For Research Use Only. Not for use in diagnostic procedures.