pJTI™ R4 Exp CMV EmGFP pA Vector

Description

pJTI™ R4 Exp CMV EmGFP pA Vector

The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green.

Ensure the Success of Your Jump-In™ Retargeting Reactions
Successful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as:

Transfection efficiency

Cell confluency

Antibiotic selection conditions

Quality and concentration of DNA

Retargeting vector to integrase vector ratio

We strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Transfection
• Format: Liquid
• Promoter: CMV
• Product Type: Mammalian Expression Vector
• Selection Agent (Eukaryotic): None
• Content And Storage: Immediately upon receipt, store vector at -20°C
• Shipping Condition: Dry Ice
• Protein Tag: Untagged
• Cloning Method: Gateway
• Quantity: 100 μg
• Vector: Jump-In Vectors
• Product Line: Jump-In™
• For Use With (Application): Stable Cell Line Development, Targeted Integration

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.