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pJTI™ R4 Exp CMV EmGFP pA Vector Non-distribution product as customer accommodation.

pJTI™ R4 Exp CMV EmGFP pA Vector

Manufacturer:  LIFE TECHNOLOGIES A14146

Catalog No. A14146


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Description

Description

The pJTI™ R4 Exp CMV EmGFP pA vector is a positive control vector for assessing the success of a retargeting reaction with a Jump-In™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector) included in the Jump-In™ parental kits, the EmGFP will be expressed and positive cells will fluoresce green.

Ensure the Success of Your Jump-In™ Retargeting Reactions
Successful retargeting of Jump-In™ parental cell lines like the the Jump-In™ GripTite™ HEK293 kit (A14150) is dependent on a variety of factors such as:

Transfection efficiency

  • Cell confluency
  • Antibiotic selection conditions
  • Quality and concentration of DNA
  • Retargeting vector to integrase vector ratio

    We strongly recommend including a retargeting reaction with the pJTI™ R4 Exp CMV EmGFP pA vector in your Jump-In™ experiment along with negative controls (no plasmid DNA, no integrase vector) so you can easily visualize the results and optimize the retargeting conditions.

    For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
  • Specifications

    Specifications

    GFP
    Transfection
    Bioproduction, cell line development for protein production, mammalian expression, protein biology, protein expression, stable cell line development, targeted integration
    pJTI R4 Exp CMV EmGFP pA Vector
    100μg
    Untagged
    Constitutive
    pJTI R4 Exp CMV EmGFP pA Vector, 100μg, GFP Tag Label, Expression Vectors Nucleic Acid Type, Mammalian Expression System, Used in Bioproduction, Cell Line Development for Protein Production, Mammalian Expression
    CMV
    None
    Jump-In™ vectors
    100μL
    Documents
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    For Research Use Only. Not for use in diagnostic procedures.