pJTI™ R4 EXP CMV-TO EmGFP pA Vector

Description

pJTI™ R4 EXP CMV-TO EmGFP pA Vector

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.

Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:

Transfection efficiency

Cell confluency

Antibiotic selection conditions

Quality and concentration of DNA

Retargeting vector to integrase vector ratio

We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.

Specifications

• Constitutive or Inducible System: Inducible
• Delivery Type: Transfection
• Inducing Agent: Tetracycline
• Promoter: CMV/TO
• Product Type: Mammalian Expression Vector
• Reporter Gene: GFP (EmGFP)
• Selection Agent (Eukaryotic): Geneticin™ (G-418)
• Content And Storage: pJTI™ R4 EXP CMV-TO EmGFP pA vector (100 μg), store at -20 °C
• Antibiotic Resistance Bacterial: Ampicillin (AmpR)
• Protein Tag: GFP (EmGFP)
• Quantity: 100 μg
• Vector: Jump-In Vectors
• Product Line: Jump-In™
• For Use With (Application): Stable Cell Line Development, Targeted Integration, Regulated Expression

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.