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pJTI™ R4 EXP CMV-TO EmGFP pA Vector Non-distribution product as customer accommodation.

pJTI™ R4 EXP CMV-TO EmGFP pA Vector

Manufacturer:  LIFE TECHNOLOGIES A15005

Catalog No. A15005


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Description

Description

The pJTI™ R4 Exp CMV-TO EmGFP pA vector is a positive control vector for assessing retargeting efficiency when retargeting a Jump-In™ T-REx™ parental cell line. When co-transfected with the integrase vector (pJTI™ R4 Int vector included in the Jump-In™ parental kits) and after antibiotic selection, the EmGFP can be inducibly expressed with doxycycline and the successfully retargeted cells will fluoresce green.

Ensure the Success of Your Jump-In™ T-REx™ Retargeting Reactions
Successful retargeting of Jump-In™ T-REx™ parental cell lines like the Jump-In™ T-REx™ HEK293 Kit is dependent on a variety of factors, such as:

  • Transfection efficiency
  • Cell confluency
  • Antibiotic selection conditions
  • Quality and concentration of DNA
  • Retargeting vector to integrase vector ratio

    We strongly recommend including a positive control retargeting reaction using the pJTI™ R4 Exp CMV-TO EmGFP pA vector in your Jump-In™ experiment, along with negative controls (no plasmid DNA, no integrase vector), so you can easily visualize the results and optimize the retargeting conditions.
  • Specifications

    Specifications

    pJTI™ R4 EXP CMV-TO EmGFP pA Vector, 100μg, Positive Control Vector for Assessing Retargeting Efficiency when Retargeting a Jump-In™ T-REx™ Parental Cell Line, Ensure the Success of your Jump-in™ T-rex™ Retargeting Reactions
    pJTI™ R4 EXP CMV-TO EmGFP pA Vector
    GFP Tag
    Protein biology, protein expression, mammalian expression, regulated expression, targeted integration, stable cell line development
    100μg
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