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ATCC pJIR750 plasmid; in E. coli
Supplier: ATCC 87015
Shuttle expression vector derived from pJIR418 (ATCC# 77387) permitting expression of antibiotic resistance in both hosts. The chloramphenicol resistance gene (catP), expressed in both Clostridium perfingens and Escherichia coli, was derived from C. perfingens and is inactivated by insertions at the EcoRV site. The vector contains the following restriction sites (approximate kb from the EcoRI site): BglI ? 0.2, 2.50; ClaI ? 4.99, 5.03; EcoRV ? 4.72; NdeI ? 0.27, 2.09; PvuI ? 0.17; PvuII ? 0.14, 6.39; SmaI ? 4.98. The order and orientation of the major features of the plasmid are: lacZ? ? EcoRI/MCS/HindIII lacZ? - pIP404 ori ? ßrep ? ßcatP ? pMB1 ori. ? Plasmid 29: 233-235, 1993.
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