Sigma Aldrich Fine Chemicals Biosciences PKH67 Green fluorescent Ce

Supplier:  Sigma Aldrich Fine Chemicals Biosciences MIDI671KT

This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2 two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2.Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.

Catalog No. 50-290-4392