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Cell Biolabs Inc Platinum-E Retroviral Packaging Cell Line, Ecotropic
Supplier: Cell Biolabs Inc RV101
The Platinum-E (Plat-E) Cell Line, a potent retrovirus packaging cell line based on the 293T cell line, was generated using novel packaging constructs with an EF1a promoter to ensure longer stability and high-yield retroviral structure protein expression. Plat-E cells stably express the gag, pol, and ecotropic envelope genes, allowing retroviral packaging with a single plasmid transfection: a cloning vector containing the gene of interest. Plat-E cells can be kept in good condition in for at least 4 months in the presence of drug selection, and can produce retroviruses with an average titer of 1 x 10^7 infectious units/mL by transient transfection. In addition, replication competent retroviruses are virtually nonexistent because only coding sequences of viral structural genes are used, avoiding unnecessary retroviral sequences. The resulting ecotropic virus can readily infect mouse or rat cells, but not cells of other species. FOR SALE TO ACADEMIC, GOVERNMENT AND NON-PROFIT LABS ONLY.
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