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Invitrogen™ pLenti6.3/V5-DEST™ Gateway™ Vector Kit
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Quantity:
1 kit
Description
Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (catalog K5330-00).
The pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
Stable expression
Long-term experiments
Accurate titer of functional virus
Flexible and versatile Gateway™ recombination cloning technology
Key Features
HiPerform™ WPRE and cPPT elements
CMV promoter
V5 epitope tag at C terminus
Blasticidin selection
Kit includes
A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ
Stbl3™ competent cells
For research use only. Not intended for any therapeutic or diagnostic use.
The pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
Key Features
Kit includes
For research use only. Not intended for any therapeutic or diagnostic use.
Specifications
Specifications
| Constitutive or Inducible System | Constitutive |
| Delivery Type | Lentiviral |
| Promoter | CMV |
| Product Type | Kit, Vector |
| Selection Agent (Eukaryotic) | Blasticidin |
| Content And Storage | Vector Kit (Box 1) pLenti6.3⁄V5-DEST™ (150 ng⁄μL): 6 μg, store at -20°C pLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄μL): 10 μg, store at -20°C One Shot™ Stbl3™ Chemically Competent E. coli (Box 2) Stbl3™ Cells: 21 x 50 μL, store at -80°C pUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°C S.O.C. Medium: 6 mL, store at -80°C |
| Cloning Method | Gateway |
| Quantity | 1 kit |
| Vector | pDEST, pLenti |
| Product Line | Gateway™, HiPerform™, ViraPower™ |
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For Research Use Only. Not for use in diagnostic procedures.
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