Invitrogen™ pLenti6.3/V5-DEST™ Gateway™ Vector Kit

Catalog No.	Quantity
V53306	1 kit

Catalog No. V53306 Supplier Invitrogen™ Supplier No. V53306

Description

pLenti6.3/V5-DEST™ Gateway™ Vector Kit

Invitrogen™ 's pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit is part of our ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (catalog K5330-00).

The pLenti6.3⁄V5-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5-DEST™ for easy recombination-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

Stable expression

Long-term experiments

Accurate titer of functional virus

Flexible and versatile Gateway™ recombination cloning technology

Key Features

HiPerform™ WPRE and cPPT elements

CMV promoter

V5 epitope tag at C terminus

Blasticidin selection

Kit includes

A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ

Stbl3™ competent cells

For research use only. Not intended for any therapeutic or diagnostic use.

Specifications

• Constitutive or Inducible System: Constitutive
• Delivery Type: Lentiviral
• Promoter: CMV
• Product Type: Kit, Vector
• Selection Agent (Eukaryotic): Blasticidin
• Content And Storage: Vector Kit (Box 1)
  pLenti6.3⁄V5-DEST™ (150 ng⁄μL): 6 μg, store at -20°C
  pLenti6.3⁄V5-GW⁄lacZ Control vector (500 ng⁄μL): 10 μg, store at -20°C
  
  One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
  Stbl3™ Cells: 21 x 50 μL, store at -80°C
  pUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°C
  S.O.C. Medium: 6 mL, store at -80°C
• Cloning Method: Gateway
• Quantity: 1 kit
• Vector: pDEST, pLenti
• Product Line: Gateway, HiPerform, ViraPower
• For Use With (Application): Viral Expression

For Research Use Only. Not for use in diagnostic procedures.