Invitrogen™ pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

Description

pLenti6.3/V5™-TOPO™ TA Cloning™ Kit

Invitrogen's™ pLenti6.3⁄V5™-TOPO™Cloning Kit is part of our ViraPower™ HiPerform™ Lentiviral Expression System (catalog K5310-00).

The pLenti6.3⁄V5™-TOPO™ -TA Cloning Kit contains the TOPO™- adapted ViraPower™ HiPerform™ lentiviral expression vector, pLenti6.3⁄V5™-TOPO™ for quick PCR-based cloning and high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5™-TOPO™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages

Stable expression

Long-term experiments

Accurate titer of functional virus

5 minute, high-efficiency TOPO™ PCR cloning in three simple steps

Key Features

HiPerform™ WPRE and cPPT elements

CMV promoter

V5 epitope tag at C terminus

Blasticidin selection

Kit includes

A lacZ vector as a positive control, pLenti6.3⁄V5-GW⁄lacZ™

Stbl3™ competent cells

For research use only. Not intended for any therapeutic or diagnostic use.

Specifications

• Cell Type: Competent Cell
• Cloning Method: TOPO-TA
• Constitutive or Inducible System: Constitutive
• Content And Storage: TOPO™ TA Cloning Reagents (Box 1)
  pLenti6.3?V5™-TOPO™ (5-10 ng⁄μL): 20 μL, -20°C
  pLenti6.3⁄V5-GW⁄lacZ™ Control vector (0.5 μg⁄μL): 20 μL, -20°C
  10X PCR Buffer: 100 μL, -20°C
  dNTP Mix: 10 μL, -20°C
  Salt Solution: 50 μL, -20°C
  CMV Forward Primer (100 ng⁄μL): 20 μL, -20°C
  V5 (C-term) Reverse Primer (100 ng⁄μL): 20 μL, -20°C
  Control PCR Template (50 ng⁄μL): 10 μL, -20°C
  Control PCR Primers (100 ng⁄μL): 10 μL, each -20°C
  Sterile Water: 1 mL
  
  One Shot™ Stbl3™ Chemically Competent E. coli (Box 2)
  Stbl3™ Cells: 21 x 50 μL, -80°C
  pUC19 control DNA (10 pg⁄μL): 50 μL, -80°C
  S.O.C. Medium: 6 mL, -80°C
• Delivery Type: Lentiviral
• Format: Kit
• Expression System: ViraPower™ HiPerform™ Lentiviral Expression Systems
• For Use With (Application): Protein Expression
• Key Functions: Viral Expression
• Product Line: HiPerform™, TOPO™, ViraPower™
• Product Type: Cloning Kit
• Promoter: CMV
• Protein Tag: V5 Epitope Tag
• Quantity: 1 kit
• Selection Agent (Eukaryotic): Blasticidin
• Selection Marker Promoter: SV40 Promoter
• Vector: pLenti, TOPO-TA Vector

For Research Use Only. Not for use in diagnostic procedures.