Invitrogen™ Human PLK4, GST Tag Recombinant Protein

Catalog No.	Quantity
PV6394	10 μg
PV6395	100 μg
PV6396	1 mg

Catalog No. PV6394 Supplier Invitrogen™ Supplier No. PV6394

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Recombinant human PLK4 (1-836) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. , Validated in LanthaScreen™ Kinase Binding Assay, Protocols available for simple use and optimized performance, Multiple pack sizes available for convenient use Protein Form: Recombinant, Fragment The gene accession number for PLK4 is NP_055079.3.

PLK4 or polo-like kinase 4 is a member of the polo family of serine/threonine protein kinases which localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. The overexpression of PLK4 triggered the simultaneous formation of multiple procentrioles around each preexisting centriole which resulting in centriole amplification and thus, Plk4-induced centriole biogenesis in human cells. The reduced Plk4 gene dosage increases the probability of mitotic errors and cancer development.

Specifications

• Accession Number: O00444
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 150mM NaCl, 0.1mM EDTA, 25% glycerol, 0.1mM PMSF, 10mM glutathione, 0.25mM DTT and no preservative; pH 7.5
• Gene ID (Entrez): 10733
• Molecular Weight (g/mol): 120.9 kDa
• Name: Human PLK4, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 1700028H20; AI385771; MCCRP2; PLK4; PLK-4; polo like kinase 4; polo-like kinase 4; SAK; serine/threonine kinase 18; serine/threonine-protein kinase 18; Serine/threonine-protein kinase PLK4; serine/threonine-protein kinase Sak; Snk akin kinase; Stk18
• Common Name: PLK4
• Gene Symbol: PLK4
• Protein Length: aa 1-836
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 1-836
• Content And Storage: Storage Buffer: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.25 mM DTT, 10 mM glutathione, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol
  
  Store at -68 to -85°C. Aliquot at first use to avoid multiple freeze-thaws.
• Assay Entry: Biochemical binding/kinetic
• Kinase Group: Serine/Threonine Kinase
• Target Entry: PLK4
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Fragment
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Drug Discovery: Cell Cycle & Proliferation, Signal Transduction