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Biotium PMA, 20mM in H2O
Supplier: Biotium 40019

PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA. The PMA-modified dsDNA cannot be amplified by PCR. The dye is designed to be cell membrane-impermeable. Thus, in a population of live and dead cells, only dead cells are susceptible to DNA modification due to compromised cell membranes. This unique feature of PMA dye makes it highly useful in selective detection of live bacteria by qPCR. A bacterial sample can be first pre-treated with PMA under light to inactivate dsDNA from dead cells. DNA is then extracted and analyzed by PCR. PMA is also available in lyophilized format. Properties: Dark red liquid; Store at -20⁰C, protect from light; Ex/Em= 510/610 nm (following photolysis and covalent attachment to DNA/RNA); MW: 511.
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