PMA qPCR live bacteria detection kit E coli (uidA) is designed for precise and reliable detection of live E coli bacteria by targeting the uidA gene using quantitative polymerase chain reaction (qPCR) Originating from advancements in molecular biology this kit employs propidium monoazide (PMA) which selectively penetrates dead cells with compromised membranes thereby allowing for exclusive amplification of DNA from live cells Its mechanism ensures that only live bacterial DNA is detected minimizing false positives associated with dead cells The kit demonstrates sensitivity in detecting bacteria within the low to moderate micromolar range and is widely used in various in vitro applications including cell culture analysis environmental microbiology and food safety testing It is frequently utilized for drug screening processes involving live bacterial assays Experimental concentrations typically depend on the specific research design