This PMAxx™-PCR kit is designed for selective detection of viable bacteria from a specific strain using PMAxx™ dye and real-time PCR. This kit provides primers for specific amplification of the Salmonella enterica invA gene. The kit has sufficient reagents for treating of 80 bacterial cultures and performing 200 PCR reactions with PMAxx™. PMAxx™ is a new and improved version of PMA designed by Biotium scientists to be a superior alternative to PMA. While PMA is generally effective at differentiating between live and dead bacteria by qPCR, it does not completely eliminate PCR products from dead cell DNA. This could potentially give false positive results. Biotium’s new dye PMAxx™ is much more effective at eliminating PCR amplification of dead cell DNA, and therefore provides the best discrimination between live and dead bacteria. Salmonella enterica is a gram-negative bacteria that causes the food-borne illness salmonellosis.