Recombinant full-length human polynucleotide kinase/phosphatase supplied lyophilized as a 100 μg vial. The protein is expressed in E. coli with an N-terminal His-SUMO tag and retains dual catalytic activity-removing 3′-phosphate groups and phosphorylating 5′-hydroxyl termini-to support studies of DNA end processing and repair. Provided with formulation and storage guidance for biochemical and structural applications.
- High purity (>90%) as determined by reducing SDS-PAGE.
- Full-length human sequence (M1-G521) corresponding to isoform 1.
- N-terminal His-SUMO tag for improved solubility and affinity purification.
- Lyophilized formulation with trehalose for enhanced stability.
- Recommended reconstitution concentration ≥100 μg/mL; freeze aliquots for long-term storage.
- Suitable for enzymatic assays, structural studies, and functional characterization of DNA repair activity.