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Invitrogen™ PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green Non-distribution product as customer accommodation.

PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green

Manufacturer:  Invitrogen™ A15905

Catalog No. A15905


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Description

Description

The PolarScreen™ Progesterone Receptor Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential progesterone receptor (PR) ligands. The kit uses insect cell-expressed, human PR ligand binding domain tagged with glutathione-S-transferase (GST) (also available separately) and a novel, high-affinity, fluorescent progesterone receptor ligand (Fluormone™ PL Green) in a homogenous, mix-and-read assay format.

This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact us at drugdiscoverytech@lifetech.com.

The PolarScreen Progesterone Receptor Competitor Assay is:

  • Complete & ready-to-use out of the box—all reagents included, just add your test compounds
  • High throughput compatible—fluorescence polarization assays in a 384-well plate
  • Convenient—components available in bulk sizes for larger screening needs

    How the Assay Works
    An N-terminal fusion of glutathione transferase to the ligand-binding domain of the human progesterone receptor (PR-LBD(GST)) is added to a fluorescently-tagged progesterone ligand (Fluormone PL Green) in the presence of competitor test compounds in microwell plates. The presence of effective competitors prevents the formation of a PL Green/PR-LBD(GST) complex, resulting in a decrease of the polarization value. The shift in polarization value in the presence of test compounds is used to determine relative affinity of test compounds for PR-LBD(GST).

    Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
  • Specifications

    Specifications

    PolarScreen™ Progesterone Receptor Competitor Assay Kit, 400 x 20μL Assays, Competitive Binding Assay, Nuclear Receptor Assay Validated Application, For Pharma & Biopharma, Target & Lead Identification & Validation
    1 Tube progesterone receptor ligand binding domain recombinant protein, 1 tube of fluormone PL green, 2 bottles of PR screening buffer, 1 tube of 1M DTT
    PolarScreen™ Progesterone Receptor Competitor Assay Kit
    For research use only
    –68 to –85/–5 to –30°C
    Pharma and biopharma, drug discovery and development, target and lead identification and validation, nuclear receptor biology, biochemical nuclear receptor assays, nuclear receptor binding
    PolarScreen™ Progesterone Receptor Competitor Assay Kit
    400 x 20μL assays
    400 x 20μL assays
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    For Research Use Only. Not for use in diagnostic procedures.