Invitrogen™ Porcine Insulin ELISA Kit

Porcine Insulin quantitates porcine Insulin in serum, plasma, supernatant.

Supplier:  Invitrogen™ ES16RBX5

Catalog No. ES16RBX5

Description

Porcine Insulin quantitates porcine Insulin in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant porcine Insulin.

Insulin is a peptide hormone secreted by beta cells of the pancreatic islets. It regulates carbohydrate, protein and lipid metabolism by enhancing membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Insulin is 51-amino acid polypeptide product produced from a precursor peptide, proinsulin. Proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds and one molecule of C-peptide. Insulin deficiency results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of b-cell origin such as insulinoma.

Specifications

• Accession Number: AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin
• Assay Sensitivity: 4 μIU/mL
• Product Type: ELISA
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: INS
• Intraassay CV: <10%
• Label or Dye: HRP
• Regulatory Status: Research Use Only
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr 20 min

• Assay Range: 4 to 300 μIU/mL
• Conjugate: Biotin
• Sample Type: Plasma, Serum, Supernatant
• Gene ID (Entrez): P01315
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Sample Volume: Plasma, 50 μL; Supernatant, 50 μL; Serum, 50 μL
• Target: Insulin
• Total Assay Time: 4 hr 45 min