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Thermo Scientific™ POROS™ AAVX RoboColumn™ Pre-packed columns
Description
RoboColumn™ chromatography products are small columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.
These RoboColumn™ columns are pre-packed with POROS CaptureSelect AAVX Affinity Resin (Cat. No. A36743), engineered to address the high selectivity and capacity requirements for downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. POROS CaptureSelect AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1 to AAV8 and AAVrh10, as well as synthetic serotypes.
POROS CaptureSelect AAVX resin enables a robust, fast, and platformable purification process for wide-ranging AAV serotypes with excellent purity and yield obtained in one step.
Features of POROS CaptureSelect AAVX resin include:
- One-step AAV purification from crude material with high purity and yield
- High specificity and capacity, maximizing yield by significantly reducing process steps
- Superior capacity maintained at high flow rates
- Excellent scalability and easier handling and packing due to rigid polymeric bead
- Non-animal derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
A wide selection of RoboColumn™ is available, including columns pre-packed with POROS ion exchange, HIC, and CaptureSelect affinity resins. All POROS high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.
RoboColumn™ is a registered trademark of Repligen Corporation.
Specifications
Specifications
| For Use With | Robotic Liquid Handling |
| Particle Size | 50 μm |
| Pore Size | 100 nm |
| Quantity | 600 μL |
| Stationary Phase | Immobilized CaptureSelect™ AAVX Ligand |
| Content And Storage | Shipped in 18% ethanol. Store at 2°C to 8°C. |
| Product Line | POROS CaptureSelect |
| Volume (Metric) | 600 μL |
| Column Type | RoboColumn |
| Purification Target | AAV |
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