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Thermo Scientific™ POROS™ Caprylate RoboColumn™ Pre-packed columns
Description
These RoboColumns are pre-packed with POROS Caprylate Mixed-Mode Chromatography Resin to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode. As a mixed-mode, weak cation-exchange resin, POROS Caprylate resin effectively removes other impurities, such as host cell proteins and a wide range of high molecular weight species. RoboColumns are small chromatography columns provided in 8-column strips. They are useful for fully automated and parallel chromatographic separations using robotic liquid handling platforms.
Features of POROS Caprylate Mixed-Mode resin include:
- Selective capture of aggregates and hydrophobic high-molecular-weight impurities
- Flow-through design that helps save buffer and material costs
- Excellent scalability and high productivity
- Wide operational range
- Non-animal-derived
POROS Caprylate Mixed-Mode resin (Cat. No. A51051) operates in flow-through mode, which helps reduce costs, especially when working with more engineered monoclonal antibody derivatives. The unique selectivity of the caprylate ligand contributes to a high yield of the targeted monomer while using established cation exchange resin buffer systems. POROS Caprylate Mixed-Mode resin is manufactured using a synthetic manufacturing process that is free of animal components.
Main characteristics
- Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
- Average particle size: 50 μm
- Ligand: octanoic acid
- Binding capacity: >20 g lysozyme/L resin
- Typical buffers: typical cation exchange buffers, pH 4.5–7.0, conductivity 10–30 mS/cm
- Clean-in-place: 0.5 M NaOH
- Formulation buffer: 0.1 M NaOH
- Mechanical resistance: 100 bar (10 MPa, 1450 psi)
Other POROS chromatography resins are also available in this column format. All POROS high performance chromatography resins have a unique pore structure that provides excellent mass transport for high capacity and resolution over a wide range of flow rates.
RoboColumn™ is a registered trademark of ATOLL GmbH.
Specifications
Specifications
| Bed Volume | 200 μL |
| Content And Storage | Store at 2–30°C. |
| Particle Size | 50 μm |
| Product Line | POROS™ |
| Quantity | 200 μL |
| Target | Monoclonal Antibodies |
| Type | Pre-packed Column |
| Stationary Phase | Caprylic Acid |
| Format | Chromatography Column |
| Column Type | High-throughput screening |
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