Thermo Scientific™ POROS™ CaptureSelect™ AAVX Affinity Resin

Description

Thermo Scientific™ POROS™ CaptureSelect™ AAVX Affinity Resin is engineered to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications.

POROS CaptureSelect AAVX Affinity Resin is engineered to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. POROS CaptureSelect AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1 to AAV8, and AAVrh10 as well as synthetic serotypes.

Features of POROS CaptureSelect AAVX resin include:

• One-step AAV purification from crude material with high purity and yield
• High specificity and capacity, maximizing yield by significantly reducing process steps
• Superior capacity maintained at high flow rates
• Excellent scalability and easier handling and packing due to rigid polymeric bead
• Non-animal derived

POROS CaptureSelect AAVX Affinity Resin enables a robust, fast, and platformable purification process for wide-ranging AAV serotypes with excellent purity and yield obtained in one step. This resin comes with complete technical and regulatory support.

Main characteristics

• Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
• Average particle size: 50 μm
• Ligand: CaptureSelect AAVX affinity ligand
• Binding capacity: >1 x 10¹³ genome copies/mL of resin
• Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
• Mechanical resistance: 100 bar (1450 psi; 10 MPa)
• Formulation buffer: 18% (v/v) ethanol

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Specifications

• Binding Capacity: >1 x 10¹³ genome copies/mL of resin
• Content And Storage: Store in refrigerator at 2°C to 8°C.
• Shipping Condition: Wet Ice
• Particle Size: 50 μm
• Form: Liquid Suspension
• Product Line: POROS™
• Quantity: 10 mL
• Type: Resin
• Column Type: Affinity