Learn More
Description
POROS CaptureSelect IgM-XL Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.
Features of this affinity matrix include:
- IgM purification from plasma and serum
- Purification of monoclonal IgM from cell cultures
- Efficient purification of IgM with high yields and retained activity
- Excellent scalability
- Non-animal derived
- High flow rates
- Cross-reactivity towards mouse and rat IgM
POROS CaptureSelect IgM-XL Affinity Matrix binds to the Fc part of IgM. The high selectivity and yields obtained using this affinity matrix enable a robust, fast, and efficient purification process with excellent purity obtained in one step.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (animal origin-free).
Main characteristics
- Matrix: cross-linked poly(styrene-divinylbenzene)
- Average particle size: 50 μm
- Ligand: POROS CaptureSelect IgM-XL affinity ligand
- Ligand coupling method: epoxide coupling
- Binding capacity: >5 g/L resin (determined with polyclonal human IgM from serum)
- Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
- Mechanical resistance: 100 bar (1450 psi, 10 MPa)
- Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Matrix | Cross-linked poly(styrene-divinylbenzene) POROS beads |
| Content And Storage | Store at 2°C to 8°C in a light-protected environment. Avoid direct exposure to sunlight. Do not freeze |
| Max. Operating Pressure | 100 bar |
| Shipping Condition | Wet Ice |
| Particle Size | 50 μm |
| Form | Resin Slurry |
| Product Line | POROS CaptureSelect |
| Quantity | 10 mL |
| Type | Affinity Matrix |
| Ligand Type | CaptureSelect IgM-XL Affinity Ligand |
| Show More |
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.