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Thermo Scientific™ POROS™ CaptureSelect™ IgM-XL Affinity Matrix
Description
POROS CaptureSelect IgM-XL Affinity Matrix is intended for the purification of human IgM, with no binding to other immunoglobulins.
Features of this affinity matrix include:
- IgM purification from plasma and serum
- Purification of monoclonal IgM from cell cultures
- Efficient purification of IgM with high yields and retained activity
- Excellent scalability
- Non-animal derived
- High flow rates
- Cross-reactivity towards mouse and rat IgM
POROS CaptureSelect IgM-XL Affinity Matrix binds to the Fc part of IgM. The high selectivity and yields obtained using this affinity matrix enable a robust, fast, and efficient purification process with excellent purity obtained in one step.
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (animal origin-free).
Main characteristics
- Matrix: cross-linked poly(styrene-divinylbenzene)
- Average particle size: 50 μm
- Ligand: POROS CaptureSelect IgM-XL affinity ligand
- Ligand coupling method: epoxide coupling
- Binding capacity: >5 g/L resin (determined with polyclonal human IgM from serum)
- Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
- Mechanical resistance: 100 bar (1450 psi, 10 MPa)
- Formulation buffer: 20% (v/v) ethanol
Specifications
Specifications
| Stationary Phase | 20% Ethanol |
| Product Line | CaptureSelect™ |
| Type | Affinity Matrix |
| Quantity | 5 mL |
| Form | Resin Slurry |
| Particle Size | 50 μm |
| Binding Capacity | >5 g/L |
| Ligand Type | IgM-XL affinity ligand |
| Matrix | Cross-linked poly(styrene-divinylbenzene) |
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