Thermo Scientific™ Power-Bind™ Streptavidin Coated Particles

Description

Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles.

Bind a variety of biotinylated ligands, including DNA/RNA, oligos, antibodies and proteins with Thermo Scientific™ Power-Bind Streptavidin Coated Particles. Particles have very high and stable biotin binding capacities and address the concerns of diagnostic test kit manufacturers and nucleic acid researchers. The microparticles reduce serum matrix effects and improve assay accuracy, resulting in low, non-specific binding of serum proteins and other interfering substances.

The use of microparticles as a solid phase support in various immunoassays and affinity purifications has been known for some time; however, achieving high activity and stable binding of solid phase ligands has been a major difficulty. Biotinylated compounds become stably bound to Power-Bind Streptavidin Coated particles after simple incubation in buffer due to the high affinity of the biotin-streptavidin interaction. The particles also function as a spacer, which improves the specific activity of the bound ligand. Monodisperse nonmagnetic particle suspensions with streptavidin covalently bound to the surface in a highly active form

Made from 0.3μm or 0.8μm OptiLink™ Carboxylate-Modified particles

High biotin-binding capacity

Extremely stable, long shelf life

Simplifies the process of binding ligands to particles

Combines the advantages of high surface area and easy, high affinity, high specific activity binding

Excellent for chemiluminescent assays, biotinylated ligand capture, and for molecular biology, immunoassay, or sample prep applications

Power-Bind Benefits Include:

• Dissociation constant (Kd 10-15 molar), easy one-step binding protocols for biotinylated ligands
• High activity of surface bound ligands can easily solve difficult coupling problems
• Simple aqueous biotinylation reactions
• Low nonspecific interactions (blocked microparticle surface)
• Highly mobile particles for membrane-based applications
• Can be used in EIA formats with biotin/enzyme detection systems
• Beneficial spacer effect of SA molecule
• A choice of 0.3μm or 0.8μm diameters for different application requirements

Recommended for:

Universal nonmagnetic bead for coupling unique ligands. Clinical diagnostics and molecular biology applications.

Specifications

• Certifications/Compliance: Includes Certificate of Analysis
• Content And Storage: 2°C to 8°C
• Description: Power-Bind Streptavidin Coated Particles
• Color: White or Colorless
• For Use With (Application): Biotinylated Ligand Binding, Diagnostic Assays
• Material: Polystyrene
• Product Type: Streptavidin-Coated Particles
• Includes: Package Insert and Certificate of Analysis
• Preservative: 0.05% Na Az
• Quantity: 5 mL
• Sample Evaluation Packs: Power-Bind evaluation samples are packaged in 1mL bottles at 1% solids (10 mg/mL). Select individual lots to evaluate.
• Specialty Packaging: Power-Bind Streptavidin Coated particles are available in different concentrations and package sizes upon request.
• Stability: Guanidinium thiocyanate; DMF and DMSO to 100% Sonication; environments PCR temperature cycling
• Diameter (Metric): 0.3 μm