Invitrogen™ pPIC6α A, B, & C Pichia Vectors

Description

pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection, high-level expression, and rapid protein purification and detection.

pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection, high-level expression, and rapid protein purification and detection. The pPIC6 A, B, & C vectors are designed for high-level secreted expression.

Both sets of vectors have the following features:

• Blasticidin resistance for direct selection of multi-copy integrants
• Inducible AOX1 promoter for high-level expression in Pichia pastoris
• C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating
• resin and detection with an Anti-His(C-term) Antibody
• pPIC6 also features:
• An É-factor secretion signal for efficient transport of proteins to the medium

Specifications

• Promoter: AOX1
• Product Type: Pichia Expression Vector
• Content And Storage: Each kit includes:
  • Expression vector in A, B, & C versions, 20 μg/vector (store at -20°C)
  • lacZ (intracellular) or HSA (secreted) control vector, 20 μg (-20°C)
  • X-33 Pichia stab (4°C)
  
  The Starter Kits also include 50 mg of Blasticidin, store at -20°C.
  
  All reagents are guaranteed stable for 6 months when properly stored.
• Protein Tag: His Tag (6x)
• Quantity: 20 μg

For Research Use Only. Not for use in diagnostic procedures.