Invitrogen™ Human PRAS40 (Phospho) [pT246] ELISA Kit

Catalog No.	Quantity
KHO0421	96 Tests

Catalog No. KHO0421 Supplier Invitrogen™ Supplier No. KHO0421

Description

ELISA

The Human PRAS40 (Phospho) [pT246] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of PRAS40 (Phospho) [pT246] in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant human PRAS40 (Phospho) [pT246]. Principle of the method A monoclonal capture antibody specific for PRAS40 (Phospho) [pT246] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, pre...

The Akt signaling pathway contributes to the regulation of apoptosis after a variety of cell death signals. AKT1S1, also known as PRAS40, is a proline-rich substrate of the kinase AKT1 and is thought to play a role in neuroprotection mediated by nerve growth factor (NGF) after transient focal cerebral ischemia. AKT1S1 is also a substrate and potential regulator of mammalian target of rapamycin (mTOR), a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Treatment with the insulin-like growth factor-1 (IGF1) can induce the phosphorylation of AKT1S1 via the PI3K/AKT signaling pathway in PC12 cells.

Specifications

• Accession Number: Q96B36
• Assay Range: 1.6-100 U/mL
• Assay Sensitivity: <0.5 U/mL
• Concentration: 1.6 U/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Kinases & Inhibitors
• Sample Type: Cell Lysates
• Substrate Type: Adaptor Proteins
• Target Species: Human
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: 1110012J22Rik; 40 kDa proline-rich AKT substrate; AI227026; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); akt1s1; Lobe; Lobel; OTTHUMP00000196756; OTTHUMP00000196760; PKB/Akt substrate 40; Pras; PRAS40; Proline-rich AKT substrate; proline-rich AKT1 substrate 11110012J22Rik; 40 kDa proline-rich AKT substrate; AI227026; AKT1 substrate 1; AKT1 substrate 1 (proline rich); AKT1 substrate 1 (proline-rich); akt1s1; Lobe; Lobel; OTTHUMP00000196756; OTTHUMP00000196760; PKB/Akt substrate 40; Pras; PRAS40; Proline-rich AKT substrate; proline-rich AKT1 substrate 1; Lobe; PRAS40
• Gene ID (Entrez): 84335
• Gene Symbol: AKT1S1
• Incubation Time: 4 hr.
• Interassay CV: 5.9%
• Intraassay CV: 4.7%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: PRAS40
• Protein Aliases: 40 kDa proline-rich AKT substrate
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: PRAS40
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr