Invitrogen™ Mouse PRDC (GREM2) ELISA Kit

Catalog No.	Quantity
EMGREM2X5	5 x 96 Tests

Catalog No. EMGREM2X5 Supplier Invitrogen™ Supplier No. EMGREM2X5

Description

ELISA

The Mouse PRDC (GREM2) ELISA quantitates Ms PDRC in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PDRC. Principle of the method The Mouse PDRC solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

GREM2 encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation.

Specifications

• Accession Number: Cktsf1b2; CKTSF1B2FLJ21195; Cysteine knot superfamily 1, BMP antagonist 2; DAN domain family member 3; DAND3; DAND3Prdc; GREM2; gremlin 2 homolog, cysteine knot superfamily (Xenopus laevis); gremlin 2, cysteine knot superfamily, homolog; gremlin 2, DAN family BMP antagonist; Gremlin1; Gremlin2; gremlin-2; Prdc; PRDCgremlin 2; Protein related to DAN and cerberμs
• Assay Range: 3.28 to 800 pg/mL
• Assay Sensitivity: 3 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 23893
• Gene Symbol: Grem2
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 33 μL, Serum 33 μL, Supernatant 100 μL
• Storage Requirements: 2°C to 8°C
• Target: GREM2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins