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Vector Biolabs CRISPR/Cas9 nuclease with FLAG tag Adenovirus (Ad-GFP-FLAG-hCas9)
Supplier: Vector Biolabs 1904
The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This adenovirus expresses a codon-optimized Cas9 nuclease with a N-terminal FLAG tag and a GFP reporter. It can be used along with guided RNA for genome-editing in cells.
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