Biotium Primary Antibody Gastric Mucin MUC5AC 2-12M1 CF740 1/EA

Supplier:  Biotium BNC740438100

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC) Its epitope is located in the N-terminal cysteine rich part of the peptide core of MUC5AC which is heavily glycosylated Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment MAb 2-11M1 reacts with the protein backbone exclusively it only reacts with fully deglycosylated MUC5AC Therefore the material under test should also be fully deglycosylated This can be achieved with standard periodate oxidation method The success of the deglycosylation can be checked with routine PAS (Periodic Acid Shiff) staining After deglycosylation the preparation should no longer be stainable with PAS reagent Only then 2-11M1 will react should MUC5AC be present This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma thus showing an expression pattern opposite to MUC2 Together with a panel of antibodies Anti-MUC5AC may be useful for differential

Catalog No. 50-004-98031