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Gibco™ Primary Mouse Hippocampal Neurons

Catalog No. A15587
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Description

Primary Mouse Hippocampal Neurons

Gibco™ Primary Mouse Hippocampal Neurons are isolated and cryopreserved from C57BL/6 embryonic day-17 mice. A flexible, ready-to-use, and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50-80%, have high neuronal purity, and are validated for functionality.

  • High viability: post-thaw viability of 50% or greater
  • High purity: greater than 98% neuronal phenotype
  • Functionally validated: tested for long-term survival, neurotransmitter response, and transduction
    • TRUSTED_SUSTAINABILITY

    Specifications

    Content And Storage Store in liquid nitrogen, vapor-phase.
    Age Fetal
    Cell Type Neural Cells
    Culture Type Mammalian Cell Culture
    Form Cryopreserved
    Species Mouse
    Tissue Hippocampus
    No. of Cells 1 Million
    Product Type Mouse Hippocampal Neuron
    Quantity 1 x 106 cells
    Remarks Mouse strain is C57BL/6
    Tested For Sterility, Viability, Mycoplasma
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    Frequently Asked Questions (FAQs)

    How were the Primary Mouse Hippocampal Neurons isolated? How specific was the dissection area (does it include the dentate gyrus, CA1, CA2, and/or CA3)? How much glutamate should I use for culturing them?

    The neurons isolated are likely from all the areas of the hippocampus you listed. The glutamate is used to establish the culture. If the media is being changed every day, the media should have glutamate supplemented through day 4 after recovery. Beyond day 4, it is not necessary and likely not preferred. Any glutamate can be used as long as it is of a higher purity such as FCC, USP, ACS, etc. grade.
    I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

    We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
    What cell types will Poly-D-Lysine support as an attachment matrix?

    The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

    For Research Use Only. Not for use in diagnostic procedures.

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