Invitrogen™ Human PRKCD (PKC delta), GST Tag Recombinant Protein

Catalog No.	Quantity
A41913	1 mg
A41911	10 μg
A41912	100 μg

Catalog No. A41913 Supplier Invitrogen™ Supplier No. A41913

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Full Length, Recombinant, Active The gene accession number for PRKCD (PKC delta) is NP_006245.2.

The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

Specifications

• Accession Number: Q05655
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 100mM NaCl, 15mM glutathione, 20% glycerol, 5mM DTT and no preservative; pH 8
• Gene ID (Entrez): 5580
• Molecular Weight (g/mol): 111.8 kDa
• Name: Human PRKCD (PKC delta), GST Tag
• pH Range: 8
• Purification Method: Purified
• Quantity: 1 mg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: 1-May; AI385711; ALPS3; CVID9; D14Ertd420e; kinase nPKC-delta; KPCD; MAY1; nPKC-delta; PKC[d]; Pkcd; PKCdelta; Prkcd; protein kinase C delta; protein kinase C delta type; Protein kinase C delta type catalytic subunit; Protein kinase C delta type regulatory subunit; protein kinase C delta variant IX; protein kinase C delta VIII; protein kinase C, delta; protein kinase C, delta IV; protein kinase C, delta V; protein kinase C[d]; SDK1; Sphingosine-dependent protein kinase-1; Tyrosine-protein kinase PRKCD
• Common Name: PKC delta
• Gene Symbol: PRKCD
• Kinase Family: PKC Kinase Family
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: Full length
• Content And Storage: -80°C, Avoid Freeze/Thaw Cycles
• Shipping Condition: Dry Ice
• Expression System: Baculovirus
• Protein Family: Kinases & Inhibitors
• Protein Form: Full Length, Recombinant, Active
• Form: Liquid
• Protein Subtype: Serine/Threonine Kinases
• Research Category: Signal Transduction

Frequently Asked Questions (FAQs)

Which kinase, similar to PCK, would be suitable for protein phosphorylationprotein?

The PRKCD (PKC delta) Recombinant Human Protein (Cat. No. A41913). This recombinant protein with a GST Tag produced on insect cells has been validated for Kinase assays thus it would be able to phosphorylate proteins.