Invitrogen eBioscience ProcartaPlex Cell Lysis Buffer
Used to lyse cells under nondenaturing conditions. Invitrogen ProcartaPlex Cell Lysis Buffer is available in 20mL units that should be stored chilled (for continual use) or frozen (for longer periods of time.) Additional protease inhibitors can be added without any difficulties.
Manufacturer: Invitrogen EPX99999000
For lysis of adherent cells, 400µL of cell lysis buffer is used for a 10cm dish. For non-adherent cells, 400µL of buffer is used per 107 cells, once they have been washed in PBS and pelleted. Buffer should be chilled and PMSF should be added to the buffer immediately prior to use.
- 20mM Tris-HCl (pH 7.5)
- 150mM NaCl
- 1mM Na2EDTA
- 1mM EGTA
- 1% Triton
- 2.5mM sodium pyrophosphate
- 1mM β-glycerophosphate
- 1mM Na3VO4
- 1µg/ml leupeptin
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