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Invitrogen™ ProcartaPlex™ Cell Lysis Buffer

Description
For lysis of adherent cells, 400μL of cell lysis buffer is used for a 10cm dish. For non-adherent cells, 400μL of buffer is used per 107 cells, once they have been washed in PBS and pelleted. Buffer should be chilled and PMSF should be added to the buffer immediately prior to use.
Buffer composition:
- 20mM Tris-HCl (pH 7.5)
- 150mM NaCl
- 1mM Na2EDTA
- 1mM EGTA
- 1% Triton
- 2.5mM sodium pyrophosphate
- 1mM β-glycerophosphate
- 1mM Na3VO4
- 1μg/ml leupeptin

Specifications
Specifications
Product Type | Buffer |
Quantity | 20 mL |
Species | Canine, Mouse, Rat, Porcine, Non-Human Primate, Human |
For Use With (Equipment) | Luminex™ Instruments |
Product Line | ProcartaPlex™ |
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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