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Invitrogen™ MEK1[pS218/pS222] Human ProcartaPlex™ Simplex Kit

Catalog No. 502884748
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Quantity:
96 Tests

Invitrogen ProcartaPlex Human MEK1[pS218/pS222] Simplex Kit enables precise analysis of the Protein of Interest.

The ProcartaPlex Human MEK1[pS218/pS222] Simplex Kit measures the level of phosphorylated MEK1[pS218/pS222] in cell lysates. It is designed to be combinable with other Simplex kits so you can create your own multiplex panel that utilizes Luminex xMAP technology for protein quantitation. When combining multiple Simplex kits (i.e., when you are not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is needed for each assay plate regardless of plex size.

For preparation of cell lysates, we recommend the use of either ProcartaPlex Cell Lysis Buffer (Cat. No. EPX-99999-000) or Cell Extraction Buffer (Cat. No. FNN0011) supplemented with 0.5 M EDTA and Halt Protease and Phosphatase Inhibitor Single to Use Cocktail 100X (Cat. No. 78440 or 78442). These buffers are not included in the kit. Depending on your cell system, other buffers can be suitable as well.

The ProcartaPlex Human MEK1[pS218/pS222] Simplex Kit is part of the ProcartaPlex Human MAPK Pathway Panel (phospho) 6plex (Cat. No. EPX060-97117-PHO), which complements the ProcartaPlex Human MAPK Pathway Panel (total) 6plex (Cat. No. EPX060-97017-901).

TRUSTED_SUSTAINABILITY

Specifications

Product Type Simplex Kit
Quantity 96 Tests
Species Human
Combinability Combinable
Bead Type Magnetic
Bead Region MEK1[pS218/pS222] [63]
Assay Sensitivity See Certificate of Analysis
Assay Range See Certificate of Analysis
For Use With (Equipment) Luminex™ Instruments
Gene MAP2K1
Gene Alias 5604
Gene ID (Entrez) MAP2K1
High-throughput Compatibility Yes
Interassay CV See Certificate of Analysis
Intraassay CV See Certificate of Analysis
Product Line ProcartaPlex™
Sample Type Cell lysates
Sample Volume Cell lysates (prediluted): 25 μL
UniProt ID Q02750
Synonym Mitogen-activated protein kinase
Content And Storage 1 vial Capture Beads (50x)
1 vial biotinylated Detection Antibody (50x)
2 vials Standard Mix (lyophilized)

Store as indicated on the Certificate of Analysis.
Shipping Condition Wet Ice
Research Area Signaling
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For Research Use Only.

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