R&D Systems™ ProDots Recombinant Human EGF Protein

Description

Supplied as ready-to-use 5 ug protein balls

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.

The ED50 for this effect is 20-100 pg/mL.

Specifications

• Accession Number: P01133
• For Use With (Application): Bioactivity
• Gene ID (Entrez): 1950
• Molecular Weight (g/mol): M.W. (Observed): 6 kDa, reducing conditions; M.W. (theoretical): 6 kDa
• Quantity: 50 μg
• Source: E. coli-derived human EGF protein Asn971 - Arg1023, with an N-terminal Met
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2°C to 8°C as supplied. 1 month at 2°C to 8°C under sterile conditions after reconstitution. 3 months (at -20°C to -80°C under sterile conditions after reconstitution.)
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Alias: beta-urogastrone, epidermal growth factor, epidermal growth factor (beta-urogastrone), HOMG4, pro-epidermal growth factor, URG, Urogastrone
• Common Name: EGF
• Gene Symbol: EGF
• Biological Activity: Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is typically 20-100 pg/mL.
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Reconstitution: For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll ProDot™ directly into cell culture medium for immediate use.
• Expression System: E. coli-derived Asn971 - Arg1023, with an N-terminal Met
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Protein: EGF