Invitrogen™ Mouse Progranulin (GRN) ELISA Kit

Catalog No.	Quantity
EMGRNX5	5 x 96 Tests

Catalog No. EMGRNX5 Supplier Invitrogen™ Supplier No. EMGRNX5

Description

ELISA

The Mouse Progranulin (GRN) ELISA quantitates Ms Progranulin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Progranulin. Principle of the method The Mouse Progranulin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Progranulin is the precursor protein of Granulin also called as Proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature Granulin. Both Granulin and smaller cleavage product of Granulin regulates cell growth. Progranulin stimulates proliferation of many epithelial cells, promotes their anchorage-independent growth and, when overexpressed, confers epithelial tumorigenicity. Progranulin/epithelin gene is multifunctional, with important constitutive roles in epithelial homeostasis, reproductive, immunological, and neuronal function. Progranulin constitutively expressed in a number of epithelia, particularly in the skin, GI tract, and reproductive system. Progranulin is expressed in immune cells in vivo and in specific neurons in the brain.

Specifications

• Accession Number: P28798
• Assay Range: 20-15000 pg/mL
• Assay Sensitivity: 20 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Transcription Factors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Acrogranin; acrogranulin; CLN11; epithelin; Epithelin precursor; Epithelin/granulin precursor; Epithelin-1; Epithelin-2; GEP; Glycoprotein of 88 Kda; GP88; granulin; Granulin A; Granulin B; Granulin C; Granulin D; Granulin E; Granulin F; Granulin G; Granulin precursor; Granulin-1; Granulin-2; Granulin-3; Granulin-4; Granulin-5; Granulin-6; Granulin-7; granulin-epithelin; granulins; GRN; Paragranulin; PC cell-derived growth factor; PCDGF; PEPI; Pgrn; Proepithelin; progranulinAcrogranin; acrogranulin; CLN11; epithelin; Epithelin precursor; Epithelin/granulin precursor; Epithelin-1; Epithelin-2; GEP; Glycoprotein of 88 Kda; GP88; granulin; Granulin A; Granulin B; Granulin C; Granulin D; Granulin E; Granulin F; Granulin G; Granulin precursor; Granulin-1; Granulin-2; Granulin-3; Granulin-4; Granulin-5; Granulin-6; Granulin-7; granulin-epithelin; granulins; GRN; Paragranulin; PC cell-derived growth factor; PCDGF; PEPI; Pgrn; Proepithelin; progranulin; Pgrn; epithelin
• Gene ID (Entrez): 14824
• Gene Symbol: GRN
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Acrogranin, Granulins
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 1 μL, Serum 1 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Progranulin
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min