Invitrogen™ Mouse Prolactin (PRL) ELISA Kit

ELISA

Supplier:  Invitrogen™ EMPRLX5

Catalog No. EMPRLX5

Description

The Mouse Prolactin (PRL) ELISA quantitates Ms PRL in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms PRL. Principle of the method The Mouse PRL solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor.

Specifications

• Accession Number: P06879
• Assay Sensitivity: 30 pg/mL
• Assay: Solid-phase sandwich ELISA
• Product Type: ELISA
• Sample Type: Plasma, Serum, Supernatant
• Sufficient For: 5 x 96 assays
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• Gene ID (Entrez): 19109
• Interassay CV: <12%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Quantity: 5 x 96 Tests
• Research Discipline: Endocrinology
• Storage Requirements: 2°C to 8°C
• Target: Prolactin
• Total Assay Time: 4 hrs 45 mins

• Assay Range: 27.43 to 20,000 pg/mL
• Calibration Range: 30 pg/mL to 20,000 pg/mL
• Conjugate: HRP
• Protein Family: Hormones & Receptors
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
• Target Species: Mouse
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene Symbol: AV290867,Gha11a1,Prl
• Intraassay CV: <10
• Label or Dye: HRP
• Regulatory Status: RUO
• Sample Volume: Plasma 20 μL, Serum 20 μL, Supernatant 50 μL
• Synonym: RP23-351I6.2, AV2908671a1, Prl
• Test Time: 1 hrs 20 mins