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Invitrogen™ ProLong™ Gold Antifade Mountant with DNA Stain DAPI
Description
ProLong Gold Antifade Mountant with DNA Stain DAPI is a mounting media that can be directly applied to fluorescently labeled cell or tissue samples on microscope slides. It comes ready-to-use and stored at room temperature—just apply a drop, add a coverslip, cure, and image. ProLong Gold Antifade Mountants come in a 2-mL dropper bottle or 10-mL bottle. It is formulated with the blue DNA stain DAPI to make your nuclear counterstaining more convenient and consistent when preparing your samples for imaging.
Achieve Excellent Refractive Index Matching Without Acrylic Resins
For high-resolution imaging applications, refractive index matching is critical. ProLong Gold Antifade Mountant will cure to an RI of 1.47 and allows you to avoid using traditional acrylic resins (i.e., fingernail polish), which can damage microscope objectives if they make contact. The refractive index determines how much the path of light is bent, or refracted, when entering a material. In microscopy, refractive index differences between specimen and mounting medium should be minimized and can therefore be reduced by using mountants with a higher RI. Optimizing the RI of your experiment will increase the resolution to provide better quality images. Depending on your sample type, thickness, imaging depth and/or staining reagents (e.g. fluorescent proteins or dyes) and whether you need a curing or non-curing mountant, choosing the right mounting media will impact image resolution and should be strongly considered for optimal experimental design.
ProLong Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins such as GFP or TagRFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, ProLong Diamond or ProLong Glass Antifade Mountants are recommended. Please note that ProLong Glass mounting media is recommended for samples > 10 microns and all immersion oil imaging applications. For immediate viewing of a sample, choose our non-curing mountant, SlowFade Gold Antifade Mountant.
Specifications
Specifications
| Quantity | 1 x 2 mL |
| Product Type | Antifade Mountant |
| Content And Storage | Store at room temperature. |
| Product Line | ProLong |
| Reagent Type | Mounting Solution, Antifade Solution |
| Volume (Metric) | 2 mL |
Frequently Asked Questions (FAQs)
They are composed of proprietary antifades and polymers in aqueous buffers.
No. If the sample cannot be sealed with a coverslip, we recommend the use of SlowFade antifade mountant.
Our ProLong Gold, ProLong Diamond, and ProLong Glass mounting media harden ('cure') by the evaporation of water. Placing the mounted samples in a humid or refrigerated environment would slow down the evaporation process ('curing').
We have not tested ProLong, ProLong Gold, ProLong Diamond, or ProLong Glass on DyLight dye-conjugated proteins or with other organic dyes, but it is likely that they will be compatible.
No. ProLong, ProLong Gold, ProLong Diamond, and ProLong Glass reagents are intended for use on fixed or fixed/permeabilized samples. Only ProLong Live Antifade Reagent (Cat Nos. P36974 and P36975) is intended for use with live cells.
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