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Innovative Research Inc Prostacyclin Kit

Catalog No. 502744227
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Prostacyclin ELISA Kit - This kit is for the quantitative determination of Prostacyclin in biofluid samples, such as serum, plasma and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . PGI2 present in samples reacts with an anti-PGI2 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, PGI2 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active PGI2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of PGI2. For RUO.

Catalog No. 50-274-4227 Supplier Innovative Research Inc Supplier No. IMLPGI2KT
May include imposed supplier surcharges.
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Prostacyclin ELISA Kit - This kit is for the quantitative determination of Prostacyclin in biofluid samples, such as serum, plasma and other biological fluids. This is a competitive inhibition ELISA in strip well format with reagents for up to 96 tests . PGI2 present in samples reacts with an anti-PGI2 monoclonal capture antibody or antigen coated and dried on a microtiter plate. Following this, PGI2 conjugated to biotin competes with the antigen in the sample to bind the capture antibody or antigen. Unbound conjugate and sample antigen is washed away and bound conjugate is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is inverse proportional to the concentration of active PGI2 in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of PGI2. For RUO.

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