Invitrogen™ Human Prostaglandin E2 Competitive ELISA Kit

Catalog No.	Quantity
ENEHPGE2	96 Tests

Catalog No. ENEHPGE2 Supplier Invitrogen™ Supplier No. EHPGE2

Description

ELISA

The Human Prostaglandin E2 (Hu PGE2) ELISA quantitates Hu PGE2 in human serum, whole blood, saliva, urine or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu PGE2. Principle of the method The Human PGE2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is inversely proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Prostaglandin E2 (PGE2) is a lipid mediator derived from arachidonic acid through the actions of cyclooxygenase (COX) enzymes and terminal prostaglandin E synthases (PGES). It plays a significant role in mediating inflammation, pain sensation, and fever by acting on four receptor subtypes: EP1, EP2, EP3, and EP4. PGE2's involvement in inflammatory responses is well-documented, particularly in diseases such as rheumatoid arthritis and osteoarthritis. Various medications, including nonsteroidal anti-inflammatory drugs (NSAIDs) and selective COX-2 inhibitors, are used to reduce PGE2 production, thereby alleviating inflammation and pain. However, these drugs can have side effects, such as gastrointestinal issues and increased risk of thrombosis. Additionally, PGE2 has a critical role in immune regulation, affecting T cell function and, by extension, the adaptive immune response. It also has a significant impact on skeletal disorders and pain through mechanisms that are still being explored.

Specifications

• Assay Range: 39.1 to 2500 pg/mL
• Assay Sensitivity: 13.4 pg/mL
• Description: PGE2 Competitive ELISA Kit
• Conjugate: AP
• Immunoassay Kit Format: Colorimetric ELISA (p-NPP substrate) with Plates
• Product Type: ELISA
• Sample Type: Saliva, Serum, Supernatant, Urine, Whole Blood
• Specificity: No significant cross-reaction with 13,14-dihydro-15-keto-PGF2a; 6,15-keto-13-dihydro-PGF1a; Thromboxane B2; 2-Arachidonoylglycerol; Anandamide; PGD2; Arachadonic acid. Less than 1% cross-reaction with PFG2a; 6-keto-PGF1a; PGA2; PGB1; Approx. 16% cross-rea
• Sufficient For: 96 microplate wells
• Target Species: Human
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 4%
• Intraassay CV: 10.7
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent, Detection Antibody, AP Conjugate, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: AP
• Measurement: Absorbance at 405 nm minus absorbance (at 570 nm)
• Precision: Dependent upon sample type and level
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Saliva 10 μL, Serum 10 μL, Supernatant 100 μL, Urine 10 μL, Whole Blood 10 μL
• Storage Requirements: -20°C
• Target: PGE2
• Test Time: 1 hrs 20 mins
• Total Assay Time: 2 hrs 45 mins

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov