Sigma Aldrich Fine Chemicals Biosciences Protein A Agarose

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 11719408001

The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic and are contaminants in Protein A isolated from S. aureus. The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times depending on the elution condition.Protein A leakage 18 ng Protein A/ml (ELISA)Regeneration the gel can be used approximately 30 timesStructure recombinant Protein A (E. coli Mr 45 000) is covalently coupled to crosslinked 6% agarose beads 3 mg Protein A (98% pure HPLC SDS-Page)/1 ml gelProtein A immobilized.

Catalog No. 50-290-6053