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Sino Biological Protein LGR5 Nanodisc 20UG

Catalog No. 5001073740
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A DNA sequence encoding the Human LGR5 (O75473) (Gly22-Ser844) was expressed with a flag tag at the N-terminusand a C-terminal polyhistidine tag followed by an AVI tag The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed This protein was prepared using our Nanodisc platform Nanodisc is a versatile tool for studying membrane proteins Using styrene-maleic acid (SMA) copolymer membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better Compared to membrane scaffold proteins (MSPs) nanodiscs SMA nanodiscs also have the advantage of preserving proteins nature by maintaining native lipids surrounded without introducing any heterologous proteins which allows studies of protein structure and functions in a native-like environment

Catalog No. 50-010-73740 Supplier Sino Biological Supplier No. 30115H94HNAB20
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A DNA sequence encoding the Human LGR5 (O75473) (Gly22-Ser844) was expressed with a flag tag at the N-terminusand a C-terminal polyhistidine tag followed by an AVI tag The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed This protein was prepared using our Nanodisc platform Nanodisc is a versatile tool for studying membrane proteins Using styrene-maleic acid (SMA) copolymer membrane proteins can be extracted directly from prokaryotic and eukaryotic expression systems in the absence of detergents to preserve the protein structure and function better Compared to membrane scaffold proteins (MSPs) nanodiscs SMA nanodiscs also have the advantage of preserving proteins nature by maintaining native lipids surrounded without introducing any heterologous proteins which allows studies of protein structure and functions in a native-like environment

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