MilliporeSigma™ Proteinase K, Lyophilized

Description

Highly active serine protease that exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA

• Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album
• Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C)
• The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment
• The Proteinase K, Lyophilized powder can be prepared as a 20mg/mL stock solution in water and stored in aliquots at -20°C
• The enzyme is also available as a ready-to-use concentrated stock solution (600mAU/mL) that is convenient for routine use in most applications
• 1mg of Proteinase K is the equivalent of 30mAU (AU = Anson unit)
• The Novagen Proteinase K products are free of detectable DNase and RNase

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Specifications

• CAS: 39450-01-6
• Content And Storage: 2°C to 8°C
• Quantity: 500 mg
• Product Type: Proteinase K