Invitrogen™ Proteinase K Recombinant Protein

Catalog No.	Quantity
PIRP87705	1 g

Catalog No. PIRP87705 Supplier Invitrogen™ Supplier No. RP87705

Description

Recombinant Protein

Animal Origin Free No DNase contamination detected by digestion of lambda DNA at 37°C for 6 hours. No RNases contamination detected by digestion of lambda DNA at 25°C for 16 hours. Host genomic DNA/RNA is no residual detected by PCR. Storage: Store the lyophilized protein at 4°C for up to 1 year and less than -20°C for long term. Lyophilized powder is recommended to dissolve in in 20mM Tris-Hcl, 30mM NaCl, 50% Glycerol, 5mM CaCl2, pH 7.2-7.4. After reconstitution, the protein solution is stable at -20°C for 1 year, at 2-8°C for up to 6 months.

Proteinase K (E.C.3.4.21.64) is an extracellular serine endoproteinase produced by Tritirachinm album Limber. Proteinase K is active in a wide range of temperatures and buffers with optimal activity between 20 and 6°C and a pH between 7.5 and 12.0. Activity is stimulated when up to 2% SDS or up to 4 M urea are included in the reaction. Calcium is important for thermostability of Proteinase K but it is not required for catalysis, therefore Proteinase K is also active in buffers containing chelating agents such as EDTA.

Specifications

• For Use With (Application): Control
• Formulation: protein with no preservative; pH 7.2-7.4
• Name: Proteinase K
• Quantity: 1 g
• Source: Yeast
• Regulatory Status: RUO
• Gene Alias: EC 3.4.21.14; Protease K
• Common Name: Proteinase K
• Biological Activity: Measured by its digestion activity. The activity is >30 U/mg One unit will digest Casein (at 37°C (pH 7.5) / minute to produce equal absorbance as 1.0 μmol of L-tyrosine.)
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: Full length Proteinase K protein
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Expression System: P. pastoris
• Form: Lyophilized
• Purity or Quality Grade: >95% by SDS-Page

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov