ATCC pRS306 phagemid; in E. coli

Supplier:  ATCC 77141

One of a series of pBluescript-based integrating vectors (ATCC 77138-77141) differing in the yeast selectable marker gene.  This is a YI-type (integrating shuttle vector ) permitting visual detection of recombinants and production of ssDNA in E. coli. It contains promoters for invitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ?) peptide.  The pRSS56, base vector was constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+).  It contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and the f1 origin of pBS(+). A fragment (1.112 kb) containing the URA3 gene was inserted into the NdeI site of pRSS56.  All ends were blunted. 

Catalog No. 50-238-1280