Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
ATCC pRS313 phagemid; in E. coli HB101
Supplier: ATCC 77142
One of a series of pBluescript-based centromere vectors (ATCC#77142-77145, 77157-77158) differing in the yeast selectable marker gene. A YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. It contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ?) peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+). A fragment (1.184 kb) containing the HIS3 gene was inserted into the NdeI site and a cassette contiaing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted.
Product Title
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.
Spot an opportunity for improvement?