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ATCC pRS424 phagemid; in E. coli
Supplier: ATCC 77105
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the number is about 20 per haploid cell. In the range of 1.5 +/? 1.7% of progeny per doubling. The order of the major features in this plasmid is: ori(fi) ? lacZ ? T7 promoter ? MCS (KpnI-SacI) ? T3 promoter ? lacI - ori (pMB1) ? ampR ? ori(2 micron) ? TRP1.
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