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Gibco™ PSC Neural Induction Medium Non-distribution product as customer accommodation. Available on GSA/VA Contract for Federal Government customers only.

PSC Neural Induction Medium

Manufacturer:  Gibco™ A1647801

Catalog No. A1647801


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Description

Description

Gibco™ PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types.

PSC Neural Induction Medium enables:

  • Rapid induction of NSCs—high efficiency neural induction in only 7 days, without EB formation
  • Scalable production of NSCs:—generate >20 million NSCs starting from 1 million PSCs
  • Generation of high quality NSCs—resulting NSCs can be cryopreserved, expanded, and differentiated

    Rapid Induction of NSCs
    PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.

    Scalable Production of NSCs
    PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune™-iPS Sendai Reprogramming Kit.

    Generation of High Quality NSCs
    Neural stem cells generated using PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes.

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  • Specifications

    Specifications

    Stem cells, stem cells (embryonic), stem cells (human), stem cells (neural), stem cells (iPS - induced pluripotent stem)
    Clinical and translational research, mammalian cell culture, primary cell culture, stem cell differentiation
    1 Each
    500mL
    Basal medium at 2 to 8°C, Supplement medium at –5 to –30°C
    PSC Neural Induction Medium, 500mL, Stem Cells, Stem Cells (Embryonic), Stem Cells (Human), Stem Cells (Neural), Stem Cells (iPS - Induced Pluripotent Stem) Cell Type, Used For Clinical and Translational Research, Stem Cell Differentiation, Mammalian Cell
    500mL Basal medium, 10mL supplement
    PSC Neural Induction Medium
    For research use only
    Basal medium: 2 to 8°C, Supplement medium: –5 to –30°C
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    For Research Use Only. Not for use in diagnostic procedures.