Gibco PSC Neural Induction Medium
PSC Neural Induction Medium
Manufacturer: Gibco A1647801
DescriptionGibco PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of PSC Neural Induction Medium does not require the intermediary step of embryoid body (EB) formation, which adds time, labor, and variability. Neural Stem Cells (NSCs) generated using PSC Neural Induction Medium have high expression of NSC markers and can be further differentiated into other neural cell types.
PSC Neural Induction Medium enables:
Rapid Induction of NSCs
PSC Neural Induction Medium simplifies the NSC generation workflow by eliminating the need to form EBs or rosettes. By removing this step, PSC Neural Induction Medium enables a more reproducible process with high efficiency neural induction in only 7 days.
Scalable Production of NSCs
PSC Neural Induction Medium is a robust medium that has been shown to generate over 20 million NSCs from 1 million PSCs. The medium has been tested with multiple hPSC lines including hiPSCs derived from use of Episomal iPSC Reprogramming Vectors and the CytoTune-iPS Sendai Reprogramming Kit.
Generation of High Quality NSCs
Neural stem cells generated using PSC Neural Induction Medium have >80% expression of Sox1, Sox2, and Nestin (NSC markers), and <1% expression of Oct4 (pluripotency marker). In addition, expanded NSCs maintained phenotype, exhibited normal karyotype, and could be differentiated into neurons, astrocytes, and oligodendrocytes.
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|Stem cells, stem cells (embryonic), stem cells (human), stem cells (neural), stem cells (iPS - induced pluripotent stem)|
|Clinical and translational research, mammalian cell culture, primary cell culture, stem cell differentiation|
|Basal medium at 2 to 8°C, Supplement medium at –5 to –30°C|
|PSC Neural Induction Medium, 500mL, Stem Cells, Stem Cells (Embryonic), Stem Cells (Human), Stem Cells (Neural), Stem Cells (iPS - Induced Pluripotent Stem) Cell Type, Used For Clinical and Translational Research, Stem Cell Differentiation, Mammalian Cell|
|500mL Basal medium, 10mL supplement|
|PSC Neural Induction Medium|
|For research use only|
|Basal medium: 2 to 8°C, Supplement medium: –5 to –30°C|
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For Research Use Only. Not for use in diagnostic procedures.