Invitrogen™ PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit

Description

The PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin.

The PureLink™ Expi Endotoxin-Free Mega Plasmid Purification Kit enables quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin. Purify up to 5 mg of high quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines. The kit utilizes a novel vacuum-assisted purification workflow that dramatically reduces purification time to approximately 2 hours.

• Simple and fast protocol—unlike other anion exchange-based endotoxin-free plasmid purification systems, a vacuum filtration method is used for lysate clarification and plasmid DNA purification. There is no waiting for gravity-flow columns, and purification typically takes 2 hours.
• High yield—isolate up to 5 mg of high quality, endotoxin-free plasmid DNA
• Endotoxin-free plasmid—produces endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells and research on gene therapy or plasmid vaccines
• Purity—ultralow levels of protein, RNA, and genomic DNA contamination
• Compatibility—purified plasmid is compatible with the Gibco™ ExpiCHO™ and Expi293™ transient mammalian expression systems
• Endotoxin-free components—includes additional endotoxin-free elution buffer, water, and plasmid storage tubes

Specifications

• Content And Storage:

  • Resuspension Buffer
  • Lysis Indicator
  • RNase A
  • Lysis Buffer
  • Precipitation Buffer
  • Endotoxin Removal Buffer
  • Wash Buffer
  • Elution Buffer
  • TE Buffer
  • Expi Mega Filtration Column
  • Expi Mega DNA Binding Column
  
  Endotoxin-free components: elution buffer, water, plasmid storage tubes

• Format: Spin Column
• Isolation Technology: Anion Exchange Resin
• Sample Type: Bacterial Culture
• Expression System: Mammalian
• For Use With (Application): Cloning, PCR
• High-throughput Compatibility: Manual Protocols
• Prep Scale: Large Volume Microbial Samples (> 10 mL)
• Product Line: PureLink™
• Product Type: Plasmid Purification Kit
• Quantity: 4 preps
• Shipping Condition: Room Temperature
• Target: Endotoxin-free Plasmid
• Test Time: 2 hr.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For research use only. Not for use in diagnostic procedures.