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Invitrogen™ PureLink™ Expi Endotoxin-Free Plasmid Purification Kits
Description
Includes
- Resuspension Buffer
- Lysis Indicator
- RNase A
- Lysis Buffer
- Precipitation Buffer
- Endotoxin Removal Buffer
- Wash Buffer
- Elution Buffer
- TE Buffer
- Expi Giga Filtration Column
- Expi Giga DNA Binding Column
- Endotoxin-free components: elution buffer, water, plasmid storage tubes
The PureLink™ Expi Endotoxin-Free Plasmid Purification Kits enable quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange resin.
The PureLink Expi Endotoxin-Free Plasmid Purification Kits enable quick and simple isolation of endotoxin-free plasmid DNA using an enhanced anion exchange membrane. In as little as 2 hours, purify up to 15 mg of high-quality plasmid DNA that is suitable for advanced transfection applications such as transfection of sensitive cells lines or genome engineering.
Advantages of the PureLink Expi Endotoxin-Free Plasmid Purification Kits include:
- Simple and fast protocol—purification typically takes 2 hours
- High yield—isolate up to 15 mg of high quality plasmid DNA
- Endotoxin-free plasmid—less than 0.1 EU/μg, ideal for sensitive applications
- High purity—ultralow levels of protein, RNA, and genomic DNA contamination
- Compatibility—suitable for use with ExpiCHO and Expi293 expression systems
- Endotoxin-free components— elution buffer, water, and plasmid storage tubes that are free of endotoxin
Simple and fast protocol
Unlike other anion-exchange-based endotoxin-free plasmid purification systems, the PureLink Expi Endotoxin-Free Plasmid Purification Kits utilize centrifugation- or vacuum-assisted filtration for lysate clarification and subsequent plasmid DNA purification. There is no waiting for gravity-flow columns and the entire purification typically takes 2 hours (including the final precipitation step).
Endotoxin-free plasmid
Purify endotoxin-free (less than 0.1 EU/μg) plasmid that is ideal for sensitive applications such as transfection of primary cells, genome engineering, and research on gene therapy or plasmid vaccines in vivo.
Compatible with ExpiCHO and Expi293 expression systems
Plasmid purified using the PureLink Expi Endotoxin-Free Plasmid Purification Kits are fully compatible with the Gibco ExpiCHO and Expi293 transient mammalian expression systems.
Superior quality with endotoxin-free components
Rest assured that the kits are of high quality and have additional endotoxin-free elution buffer, water, and plasmid storage tubes for added peace of mind.
Specifications
Specifications
| Content And Storage | • Resuspension Buffer |
| Format | Spin Column |
| Isolation Technology | Anion Exchange Resin |
| Sample Type | Bacterial Culture |
| Expression System | Mammalian |
| For Use With (Application) | Cloning, PCR |
| High-throughput Compatibility | Manual Protocols |
| No. of Reactions | 2 Preps |
| Prep Scale | Large Volume Microbial Samples (> 10 mL) |
| Product Line | PureLink |
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Frequently Asked Questions (FAQs)
The anion exchange resin used in PureLink HiPure plasmid purification kits produce plasmid DNA with inherently low endotoxin levels of 0.1-1.0 EU/µg. To add to this, PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits contain endotoxin-free components, and a proprietary Endotoxin Removal Buffer that further enhances endotoxin depletion, resulting in plasmid DNA that is consistently endotoxin-free (< 0.1 EU/µg) .
No. Endotoxin Removal Buffer (ER) in PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits is not sold separately.
The PureLink Expi Endotoxin-Free Mega/Giga plasmid purification kits can produce endotoxin-free (< 0.1 EU/µg) advanced transfection-grade plasmid DNA.
Safety and Handling
For Research Use Only.
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