Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit

Description

The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times.

The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times. The PureLink™ HiPure Expi Plasmid Megaprep Kit typically isolates 4 mg of high quality, ultrapure plasmid DNA with inherently low endotoxin levels from 500 mL of bacterial culture.

Advantages of the PureLink™ HiPure Expi Plasmid Megaprep Purification Kit include:

• High yield—isolate over 5mg of high quality plasmid DNA from a single purification using 1L of bacterial culture volume
• Purity—inherently low endotoxin levels (typically 0.1–1.0 EU/μg) and A260/280 >1.8, making it ideal for mammalian cell transfection
• Simple and super-fast protocol—with vacuum assisted protocol, typical plasmid isolation takes as little as 90 minutes

HiPure Expi anion exchange chromatography for plasmid purification
PureLink™ HiPure Expi plasmid purification kits use a novel, proprietary anion-exchange resin to enable the purification of the highest levels of transfection-grade plasmid DNA. The enhanced resin combines large capacity with fast flow rate, high resolution, and efficient endotoxin removal. Plasmid preparations are typically completed in less than 2 hours with purity equal to two CsCl gradients. PureLink™ HiPure Expi plasmid purification kits enable the quickest process for obtaining the highest amount of transfection-grade plasmid DNA.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

• Content And Storage: • Equilibration Buffer
  • Resuspension Buffer
  • Lysis Buffer
  • Precipitation Buffer
  • RNase A
  • Wash Buffer
  • Elution Buffer
  • TE Buffer
  • Expi HiPure Mega Filtration Column
  • Expi HiPure Mega DNA Binding Column
  
  Store all components at room temperature upon receipt.
• Format: Kit
• Isolation Technology: Anion Exchange Resin
• For Use With (Application): Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
• High-throughput Compatibility: Manual Protocols
• No. of Reactions: 4 Preps
• Prep Scale: 4 mg plasmid DNA
• Product Line: PureLink™
• Product Type: Plasmid MegaPrep Kit
• Quantity: 4 Preps
• Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
• Target: Plasmid DNA
• Test Time: 2 hr.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.