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New England Biolabs, Inc. PURExpress® In Vitro Protein Synthesis Kit – 100 reactions
Supplier: New England Biolabs, Inc. E6800L
A rapid method for gene expression analysis, PURExpress is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. With minimal nuclease and protease activity, the PURExpress system preserves the integrity of DNA and RNA templates/complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
- Defined system with his-tagged proteins for transcription/translation; Ribosome is not his-tagged
- Minimal nuclease and protease activity for stability of synthesized protein and encoding target
- Templates can be either plasmid DNA, linear DNA or mRNA
- Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820)
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